MOLECULAR DEVELOPMENTAL NEUROBIOLOGY

Lardelli Laboratory - CMGD Adelaide Telephone: +61 8 8303 3212 Facsimile: + 61 8 8303 7534 Email: michael.lardelli@adelaide.edu.au

Research Focus

Michael Lardelli and his team analyse the molecular pathology of Alzheimer's disease using the zebrafish. The group is currently focussed on the central role of the presenilin genes in this disease:

• Truncations of presenilin proteins and their effects on presenilin function in development and disease
• Molecular misreading in presenilin transcripts

Recent publications

Australian Collaborators

• Prof Ralph Martins and Dr Giuseppe Verdile, The McCusker Foundation for Alzheimers Disease Research Hollywood Private Hospital, Perth, WA. Investigation of the role of presenilin genes in the molecular pathology of Alzheimer's disease.
• Dr Ian Musgrave, Dept of Pharmacology, The University of Adelaide. Using animal models to investigate oxidative stress in Alzheimer's disease.
• Dr Stephen Wood, Child Health Research Institute. Adelaide. Expression of usp9 during zebrafish embryo development
• Dr Yeesim Khew-Goodall, Institute of Medical and Veterinary Science, Adelaide. The role of Pez in embryo development
• Dr Chris Hahn, Hanson Institute, Adelaide. The role of VasGAP in Tumourigenesis
• A/Prof Robert Richards, School of Molecular and Biomedical Science, The University of Adelaide. The role of huntingtin in zebrafish embryo development
• Dr Daniel Peet, School of Molecular and Biomedical Science, The University of Adelaid. The function of FIH-1 in zebrafish embryo development
• Drs Simon Koblar and Martin Lewis, School of Molecular and Biomedical Science, The University of Adelaide. The role of Nxf in embryo development and epilepsy
  
• ARC/NHMRC Research Network in Genes and Environment in Development (NGED)
  
  

International Collaborators

• Drs Andy Oates and Carl-Philipp Heisenberg, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. The role of agtrl1 in somitogenesis and epiboly.
  Dr Randall Moon, Howard Hughes Medical Institute, University of Washington, Seattle, Washington, USA. Control of beta-catenin activity by presenilins in zebrafish.
  

Publications (since 2000)

Scholpp, S., Groth, C., Lohs, C., Lardelli, M., Brand, M. (2004) Zebrafish fgfr1 is a member of the fgf8 synexpression group and is required for fgf8 signalling at the midbrain-hindbrain boundry. Development, Genes and Evolution 214: 285-95

Tucker, B., Richards, R., Lardelli, M. (2004) Expression of three zebrafish orthologs of human FMR1-related genes and their phylogenetic relationships. Development, Genes and Evolution 214: 567-74

Camp, E.C., Hope, R., Kortschak, R.D., Cox, T.C., Lardelli, M.T. (2003) Expression of three spalt (sal) gene homologues in zebrafish embryos. Development, Genes and Evolution. 213:35-43

Camp, E., Badhwar, P., Mann, G.J., Lardelli, M. (2003) Expression analysis of a tyrosinase promoter sequence in zebrafish. Pigment Cell Research 16: 117-26

Lardelli, M. (2003) Something fishy in 21 st century genomics. Today's Life Science 15:20-3

Lardelli, M. (2003) The evolutionary relationships of zebrafish genes tbx6, tbx16/spadetail and mga. Development, Genes and Evolution 213: 519-22

Nornes, S., Groth, C., Camp, E., Ey, P., Lardelli, M. (2003) Developmental control of Presenilin1 expression, endoproteolysis and interaction in zebrafish embryos. Experimental Cell Research 289: 124-32

Groth, C. Lardelli, M.T. (2002) The structure and function of vertebrate fibroblast growth factor receptor 1. International Journal of Developmental Biology 46: 393-400

Groth, C., Nornes, S., McCarty,R., Tamme, R., Lardelli, M. (2002). Identification of a second presenilin gene in zebrafish with similarity to the human Alzheimer's disease gene presenilin2. Development Genes and Evolution 212:486-90

Lardelli, M. (2002) Nonspecific, nested suppression PCR method for isolation of unknown flanking DNA ("Cold-start method") in Methods in Molecular Biology, Vol. 192: PCR Cloning Protocols, 2nd. Ed., pages 285-99, Edited by B.-Y. Chen and H. W. Janes, Humana Press Inc., Totowa, N.J., U.S.A.

Lardelli, M. (2002) Generation and PCR-screening of bacteriophage lambda sublibraries enriched for rare clones (the "sublibrary method"). In Methods in Molecular Biology, Vol. 192: PCR Cloning Protocols, 2nd. Ed., pages 391-9, Edited by B.-Y. Chen and H. W. Janes, Humana Press Inc., Totowa, N.J., U.S.A.

Tamme, R., Wells, S., Conran, J.G. and Lardelli, M. (2002) The identity and distribution of neural cells expressing the mesodermal determinant spadetail. BMC Developmental Biology 2: 9

Camp, E., Lardelli, M. (2001) Tyrosinase gene expression in zebrafish embryos. Development genes and evolution 211: 150-153

Holland, L.Z., Tamme, R., Kortschak, R.D., Abi Rached, L., Holland, N.D., Inoko, H., Shiina, T., Burgtorf, C., Lardelli, M. (2001) Characterization and developmental expression of the amphioxus homolog of Notch (AmphiNotch): Evolutionary conservation of multiple expression domains in amphioxus and vertebrates. Developmental Biology 232:493-507

Jönsson, J.I., Xiang, Z., Pettersson, M., Lardelli, M., Nilsson, G. (2001) Distinct and regulated expression of Notch receptors in hematopoietic lineages and during myeloid differentiation. European Journal of Immunology 31:3240-7

Kortschak, R. D., Tamme, R. and Lardelli, M. (2001) Evolutionary analysis of vertebrate Notch genes. Development genes and evolution 211:350-4

Tamme, R., Mills, K, Rainbird, B., Nornes, S., Lardelli, M. (2001) Simple, directional cDNA cloning for in situ transcript hybridisation screens. BioTechniques 31:938-42, 944, 946

Tamme, R., Camp, E., Kortschak, R.D., Lardelli, M. (2000) Non-specific, nested suppression PCR method for isolation of unknown flanking DNA. BioTechniques 28: 895-902.